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Newswise — People with epilepsy experience uncontrolled seizures that can impair quality of life and cause stigma that leads to social isolation. The neurological condition can limit some activities most people take for granted, such as sustaining work or operating a vehicle. Researchers at the University of Pennsylvania and funded by the National Institute of Biomedical Imaging and Bioengineering (NIBIB) have developed a non-invasive brain imaging technique for a class of patients whose epilepsy symptoms do not respond to drug treatment and who would otherwise be poor candidates for seizure-relieving surgeries. Epilepsy affects approximately 65 million people worldwide. Seizure-controlling medications are ineffective in approximately one third of these patients. Alternatively, doctors can perform surgery to remove the source of the seizures, which often is a lesion, or scarring, within the brain. Conventional imaging procedures, such as magnetic resonance imaging (MRI) and positron emission tomography (PET) help surgeons identify and remove brain lesions. Approximately one third of those with drug-resistant epilepsy, however, do not have lesions that conventional brain imaging can detect. Now, researchers report a new specialized imaging technique that can trace the location of seizures that are not detected with conventional MRI or PET. The imaging technique, known as glutamate chemical exchange saturation transfer (GluCEST), was developed in the laboratory of senior author Ravinder Reddy, Ph.D., a professor of radiology and director of the University of Pennsylvania’s Center for Magnetic Resonance and Optical Imaging. The work is reported in the October issue of Science Translational Medicine. “This non-invasive MRI technique images distinct patterns and changes in glutamate levels in brain structures that could be indicative of neurological disorders” explains Richard Conroy, Ph.D., director of the NIBIB Division of Applied Science and Technology. “The research team is pursuing the tracking of glutamate because it is a key amino acid involved in transmitting signals between neurons, making it a potential marker for identifying the region of the brain where abnormal firing of neurons could cause epileptic seizures.” Normally, glutamate acts as an excitatory signal that relays messages through the brain and then quickly dissipates. In previous studies, researchers showed that glutamate does not dissipate in animals and humans with epilepsy, resulting in a build-up of glutamate that causes overstimulation and the onset of seizures. The specialized MRI technique relies on unique chemical properties of glutamate that permit water molecules surrounding the chemical to be visualized at a very high resolution. The increased signal from the surrounding water molecules indicates increased levels of glutamate. The researchers studied how GluCEST imaging detects glutamate in the hippocampus. The hippocampus is a bilateral area of the brain involved in spatial navigation and the conversion of short-term thoughts into long-term memories. Seizures often originate in the temporal lobes of the brain, which include the right and left halves of the hippocampus. In four patients with drug-resistant epilepsy, GluCEST imaging enabled the researchers to detect consistently higher levels of glutamate in the side of the hippocampus where the epileptic seizures originated. The researchers confirmed the results with electroencephalography, which identifies the side of the brain emitting irregular waves as a seizure is occurring. In 11 healthy individuals tested for comparison, the GluCEST signal for glutamate was the equivalent, or normal, in either side of the hippocampus. “The demonstration that GluCEST can localize hot spots of increased glutamate is a promising step towards improved treatment,” says Kathryn Davis, M.D., an assistant professor of neurology at the Perelman School of Medicine at the University of Pennsylvania and lead author of the study. “Finding the epileptic foci in a specific brain region gives clinicians critical information to guide targeted therapies that have the potential to control seizures in patients that currently do not have treatment options.” The researchers are optimistic that the GluCEST imaging technique offers patients the possibility for expanded epilepsy treatments, which could include surgery or laser ablation therapy. Neurostimulation—electrical stimulation similar to therapy for patients with Parkinson’s disease—may also be able to reduce abnormal excitation that produces erratic movements and seizures. The research was supported by the National Institutes of Health through a grant (EB015893) from NIBIB and a grant from the National Institute of Neurological Disorders and Stroke. Additional funding was provided by a McCabe Pilot Award and a University of Pennsylvania Center for Biomedical Image Computing and Analytics Seed Award.
Newswise —  University of Oregon scientists have found that strength in numbers doesn't hold true for microbes in the intestines. A minority population of the right type might hold the key to regulating good health. The discovery, based on research using zebrafish raised completely germ free, is reported in a paper published in the Nov. 11 issue of Cell Host & Microbe. The findings provide a path to study the function of each bacterial species in the gut and to eventually, perhaps, predict and prevent disease, says lead author Annah S. Rolig, a postdoctoral researcher in the UO's Institute of Molecular Biology. In the project, researchers watched for immune response as isolates of species of bacteria, normally associated with healthy zebrafish, were introduced one at a time and in combination into previously germ-free intestines of the fish. In a telling sequence, one bacterial species, Vibrio, drew numerous neutrophils, which indicated a rapid inflammatory response in one fish. Another species, Shewanella, inserted into a separate germ-free fish barely attracted an immune response. In a third germ-free fish, both species were introduced together and assembled with a ratio of 90- percent Vibrio to 10-percent Shewanella. The inflammatory response in the third fish was completely controlled by the low-abundance species. "Until now, we've only been able to capture proportional information, like you'd see displayed in a pie graph, of the makeup of various microbiota, in percentages of their abundance," Rolig said. "Biologists in this field have typically assumed an equal contribution based on that makeup." Low counts of a bacterial species generally have been discounted in importance, but slight shifts in the ratios of abundant microbe populations have been thought to have roles in obesity, diabetes and inflammatory bowel diseases such as Crohn's disease. That thinking is now changing, Rolig said. "The contribution of each bacterium is not equal. There is a per-capita effect that needs to be considered." The keystone - an important participant that functions to regulate a healthy microbiota - may reside in low-abundant bacterial species. The research team found through additional scrutiny that these species secreted molecules - for now unidentified - that allowed them to dampen the immune response to the whole community. "Now we've shown that these minor members can have a major impact. If we can identify these keystone species, and find that in a disease state one species may be missing, we might be able to go in with a specific probiotic to restore healthy functioning," said Rolig, who also is a scientist in the National Institutes of Health-funded Microbial Ecology and Theory of Animals Center for Systems Biology, known as the META Center, at the UO. To develop a model to capture per-capita contributions of microbes in a population, Rolig and her co-authors -- biology graduate student Adam R. Burns, microbiologist Brendan Bohannan of the Institute of Ecology and Evolution and biologist Karen Guillemin, director of the META Center -- turned to UO physicist Raghuveer Parthasarathy. His math-driven model, detailed in the paper, provides formulas that predict collective inflammatory responses of combinations of bacteria. "I'm really proud of this paper because it exemplifies an achievement of one of the major goals of the META Center for Systems Biology, namely to provide a predictive model of how host-microbe systems function," Guillemin said. "This experimental and modeling framework could be readily generalized to more complex systems such as humans, for example to predict disease severity in individuals with inflammatory bowel disease based on the pro-inflammatory capacity of their gut microbes as assayed in cell culture." ### The National Institutes of Health supported the research through grants P50GMO98911 to support the META Center, IF32DK098884 for a postdoctoral fellowship to Rolig and P01HD22486 that supports the UO's zebrafish facility. Sources: Annah S. Rolig, postdoctoral research associate, UO Institute of Molecular Biology, 541-346-5999, arolig@uoregon.edu, and Karen Guillemin, professor of biology and director of the META Center for Systems Biology, 541-346-5360, guilleman@molbio.uoregon.edu Note: The UO is equipped with an on-campus television studio with a point-of-origin Vyvx connection, which provides broadcast-quality video to networks worldwide via fiber optic network. There also is video access to satellite uplink and audio access to an ISDN codec for broadcast-quality radio interviews.
Newswise —  A diet rich in vitamin C could cut risk of cataract progression by a third, suggests a study being published online today in Ophthalmology, the journal of the American Academy of Ophthalmology. The research is also the first to show that diet and lifestyle may play a greater role than genetics in cataract development and severity. Cataracts occur naturally with age and cloud the eye’s lens, turning it opaque. Despite the advent of modern cataract removal surgery, cataracts remain the leading cause of blindness globally.1 Researchers at King’s College London looked at whether certain nutrients from food or supplements could help prevent cataract progression. They also tried to find out how much environmental factors such as diet mattered versus genetics. The team examined data from more than 1,000 pairs of female twins from the United Kingdom. Participants answered a food questionnaire to track the intake of vitamin C and other nutrients, including vitamins A, B, D, E, copper, manganese and zinc. To measure the progression of cataracts, digital imaging was used to check the opacity of their lenses at around age 60. They performed a follow-up measurement on 324 pairs of the twins about 10 years later. During the baseline measurement, diets rich in vitamin C were associated with a 20 percent risk reduction for cataract. After 10 years, researchers found that women who reported consuming more vitamin C-rich foods had a 33 percent risk reduction of cataract progression. Genetic factors accounted for 35 percent of the difference in cataract progression. Environmental factors, such as diet, accounted for 65 percent. These results make the study the first to suggest that genetic factors may be less important in progression of cataract than previously thought.How vitamin C inhibits cataract progression may have to do with its strength as an antioxidant. The fluid inside the eye is normally high in vitamin C, which helps prevents oxidation that clouds the lens. More vitamin C in the diet may increase the amount present in the fluid around the lens, providing extra protection. Researchers noted that the findings only pertain to consuming the nutrient through food and not vitamin supplements. “The most important finding was that vitamin C intake from food seemed to protect against cataract progression,” said study author Christopher Hammond, M.D., FRCOphth, professor of ophthalmology at King’s College London. “While we cannot totally avoid developing cataracts, we may be able to delay their onset and keep them from worsening significantly by eating a diet rich in vitamin C.”
Newswise — Obese young people can still turn their chances of developing life threatening illness around if they change before middle age, says new research. The study looked at the body mass index (BMI) of people when they were young and compared it to when they were middle aged to see whether it affected their risk of heart attack, stroke or diabetes. Men who had high BMI levels at 21, but had lowered their BMI by the time they were 50, had similar or lower rates of diabetes as people who were normal weight when younger, the results showed. In a unique approach, the study used the records of men’s military service, which recorded their BMI at 21, as well as participant recall and followed up with them 30 years later. Lead research Professor Christopher Owen from St George’s University of London said the effects of high BMI early in life may be reversible. “Even in men who carried out UK National Service and were relatively thin in early life compared to more recent men, higher levels of fatness in early adult life appear to be associated with later diabetes,” he said. “However, effects of early body mass appear to be reversible by subsequent weight loss. These findings have important implications for Type 2 diabetes prevention, especially in more recent adults with high levels of obesity.” But the study, which examined almost 5000 men, found that a higher BMI earlier in life did not impact on the risk of heart attack or stroke. However, men who were obese when they were 50 had increased chances of suffering a heart attack, stroke or diabetes. Obesity is the biggest risk factor for type 2 diabetes and over 4 million people in the UK are at high risk of developing the condition.
Newswise —  Resistance and endurance exercises affect the body very differently. These differences suggest that adapting to exercise involves many processes, but scientists have observed that one gene in particular, peroxisome proliferator-activated receptor-gamma coactivator PGC-1α, controls many of them. New research in Physiological Reports shows that although both resistance and endurance exercises activate the PGC-1α gene, the adaptation processes stimulated are not the same and depend on the type of exercise. Proteins run the body: They turn processes on or off or speed them up or slow them down. The body has many different proteins, and the instructions to make them are written on sections of DNA, referred to as genes. Different genes code for different proteins, but different proteins can also come from the same gene. Called isoforms, these proteins are produced when only part of the gene’s code is read. The PGC-1α protein turns on other genes. Several studies have shown that isoforms of PGC-1α exist and that the isoform produced depends on the exercise. In this new study, researchers at the University of Jyväskylä in Finland comprehensively examined the isoforms present shortly after exercising and the genes those isoforms turned on. Samples were taken from the thigh muscles of healthy men after they performed high-intensity resistance exercises or moderate-intensity endurance exercises. The researchers found that both endurance and resistance exercises produced isoforms PGC-1α exon 1b, PGC-1α exon 1b’ and truncated PGC-1α, while only endurance exercise produced PGC-1α exon 1a isoform. Endurance exercise activated genes that stimulated growth of new blood vessels and increased endurance. Resistance exercise also activated a gene that promoted blood vessel growth, along with a gene that encouraged muscle growth. “Our results support that gene expression responses of PGC-1α isoforms may have an important role in exercise-induced muscle adaptations,” the researchers stated. The study “PGC‐1 isoforms and their target genes are expressed differently in human skeletal muscle following resistance and endurance exercise” is published in the October issue of Physiological Reports, a joint journal of the Physiological Society and American Physiological Society.
Newswise — UC Berkeley biologists have discovered the switch that triggers the power kick sperm use to penetrate and fertilize a human egg, uncovering a possible source of male infertility but also a potential target for contraceptives that work in both men and women. The switch is a protein receptor that responds to the female sex hormone progesterone, which is released by the egg or oocyte, the ultimate goal toward which sperm swim. Thousands of these receptors sit on the surface of a sperm’s tail and when the sperm gets close to the egg, the hormone activates the receptor and triggers a cascade of changes that make the tail snap like a whip, powering the sperm into and hopefully through the cells protecting the egg. “If the receptor protein doesn’t recognize progesterone, you would be infertile,” said Melissa Miller, a postdoctoral fellow at both UC Berkeley and UC San Francisco and the first author of a paper reporting the discovery. “This gives us an understanding of another pathway that is involved in human sperm activity.” A drug that inactivates this newly discovered receptor, however, might make a good “unisex” contraceptive – one that could be used by either sexual partner. “What’s really cool is that we have an actual target for unisex contraceptive development,” Miller said. “If you can stop progesterone from inducing a power stroke, sperm are not going to be able to reach or penetrate the oocyte.” While there are other possible targets for a contraceptive that would prevent the initiation of the power stroke, called hyperactivation, or the simultaneous release of enzymes that cut through the protective layer around the egg, “this is one of the better options we have for a unisex contraceptive,” she said. Senior author Polina Lishko, a UC Berkeley assistant professor of molecular and cell biology, noted that many tissues – the brain, the lungs, smooth muscle – contain related progesterone or steroid receptors that may work in a similar manner to trigger major changes in tissues. “Now that we know the players, the next step is to look in other tissues that express these proteins to see whether progesterone acts on them in a similar manner to affect pain threshold adjustment in pain sensing neurons, surfactant production in the lungs or the excessive smooth muscle contractions found in asthma,” she said. “This may be a universal pathway in all cells.” Miller, Lishko and their colleagues will publish their findings in the March 17 “Fast Release” issue of the journalScience. Few known causes of male infertility Today, doctors are unable to determine the cause of nearly 80 percent of all cases of male infertility, in part because little is known about the many molecular steps involved in the production of sperm and its interactions with the egg. Sperm may be to blame in half of all cases of infertile couples. Yet because the U.S. government forbids the use of federal funds for research that brings eggs and sperm together in the same dish, little research has been done on how egg-sperm interactions lead to infertility. And until five years ago, it was very difficult to study the inner workings of sperm – the body’s smallest cell – with ordinary lab techniques. The new discovery comes thanks to techniques that Lishko and her colleague Yuriy Kirichok developed over the past five years at UCSF and UC Berkeley. The techniques allow them to stick electrodes on a sperm’s tail and record its reactions to hormones, key to probing the molecular cascades that govern sperm behavior. That technique led to their discovery that a large receptor on sperm tails – a calcium channel dubbed CatSper – is activated by progesterone from the egg. Progesterone unlocks the channel gate, letting electrically charged calcium atoms flood into the cell. This leads to a biochemical cascade that readies the sperm cell for its last-ditch effort to fertilize the oocyte. Miller and Lishko suspected, however, that progesterone was not acting directly on the calcium channel, but on some other receptor that, in turn, activated the calcium channel. That proved to be the case. They showed that progesterone actually binds to a previously mysterious enzyme called ABHD2, which is found at high levels in sperm. Once progesterone binds to the enzyme, which sits on the surface of the sperm, it removes a lipid (2AG) that has been inhibiting the calcium channel. Released of inhibition, CatSper opens the gate to calcium ions and eventual sperm activation. The inhibitor of the calcium channel CatSper is probably there for a good reason: to prevent sperm from prematurely sprinting toward the egg and using up their limited supply of energy, Miller said. Marathon or team sport? “People tend to think of fertilization as like a marathon, where the fastest, most powerful sperm is going to win,” Miller said. “We think of it like the Tour de France, where the riders in front are blocking the wind for the actual winner. Fertilization is a team sport, where the first sperm clear the way, expending their energy to break through the barrier cells, so that the slow and steady guy can get into the oocyte.” The study also sheds light on a long-standing mystery about steroids like progesterone: why they appear to act in two distinctly different ways. As a sex hormone, progesterone usually triggers a cascade of events in the cell that alter the expression of genes in the nucleus, a process that can take days. But sometimes progesterone causes immediate changes in the cell – something called non-genomic steroid signaling – that evidently use a quicker process than gene expression. Lishko, who studies the sperm of rats, mice, bulls and boars as well as human to understand how fertilization works across different species, says that sperm are a perfect system in which to study non-genomic steroid signaling, since the genes in sperm are silenced and the normal type of steroid signaling is not present. As she and her colleagues uncover the basics of steroid signaling in sperm, the same process can then be studied in many other types of cells, she said. The research was supported by the National Institutes of Health and by Pew Scholars and Alfred P. Sloan Awards to Lishko. Lishko, Miller and Kirichok have filed a patent on usage of ABHD2. Aside from Miller, Lishko and Kirichok, other authors of the paper are Nadja Mannowetz, Anthony Iavarone, Rojin Safavi, Rose Hill and Diana Bautista of UC Berkeley, Elena Gracheva of Yale University and James Smith of UCSF.   Healthy sperm employ a regular sinusoidal tail motion when swimming, unlike the whiplike motion they use when they reach the egg.
Newswise — A University of California, Irvine scientific team led by infectious diseases researchers Philip Felgner and Aaron Esser-Kahn has received $8 million from the U.S. Department of Defense’s Defense Threat Reduction Agency to help develop a new vaccine for Q fever. Caused by the Coxiella burnetii bacterium, Q fever is a highly infectious agent common among livestock. It has a history of being aerosolized for use in biological warfare and is considered a potential bioterrorism weapon. Q fever is also a public health threat; a 2007-10 outbreak in the Netherlands affected thousands of people. Symptoms include high fever, nausea, severe headache and abdominal pain. It is rarely fatal. “The current vaccine for Q fever is effective but has severe side effects that limit its widespread use,” said Felgner, an adjunct professor of medicine at UCI. “It’s a high priority that this vaccine be administered to members of the armed forces. Consequently, the military is interested in developing an alternative protective vaccine that’s safer and does not cause adverse reactions.” Felgner will use an approach he pioneered at UCI to create whole proteome microarrays to discover immune response-activating antigen proteins that may be effective as a vaccine. Additionally, he’ll collaborate with Esser-Kahn, assistant professor of chemistry, whose group will develop synthetic agents that can boost and control the immune response to these proteins. Felgner said this dual method may be applicable in creating more vaccines important to the military and general public health, adding that this is an opportunity for the Department of Defense to test these methods for their potential use against other infectious diseases. After identifying the target proteins, Felgner will work with the U.S. Army Medical Research Institute of Infectious Diseases at Fort Detrick in Maryland on next-stage animal studies of a candidate vaccine. The project is a successful outgrowth of the Pacific Southwest Regional Center of Excellence, one of only 11 National Institutes of Health-funded research sites dedicated to countering threats from bioterrorism agents and emerging infectious diseases. UCI received $85 million for this effort, which was led by Dr. Alan Barbour, professor of microbiology & molecular genetics. The federal program ended in 2015.  
Credit Newswise — UCLA researchers have found that space-mapping neurons – the GPS system in the brain - have a strong dependence on what is being looked at when triangulating location, a finding that resolves a neurological mystery that has vexed scientists for more than four decades. This also expands on an earlier finding that neurons responsible for creating spatial maps react differently in virtual reality than they do in the real-world environments. Researchers again used rats in a virtual reality environment to test the long-debated theory of whether landmarks are necessary or whether that region of the brain is also counting steps or directional movement to determine location, said Mayank Mehta, a UCLA professor of neurology, physics and astronomy, and neurobiology in the UCLA College and the study’s senior author. The study, which appears today in the peer-reviewed journal Cell, showed that many neurons were firing selectively only when rats were looking at certain landmarks on screens, either in the real or in the virtual reality environment. “This part of the brain, the hippocampus, has neurons that fire in specific places. If I’m walking around a room, some neurons fire near the door, others around the middle of the room, and they all form a map of space in the brain,” said Mehta, who also is director of a W.M. Keck Foundation neurophysics center. “Where does this map come from? The classic idea was there are two possible mechanisms. One hypothesis is that neurons triangulate distances with respect to visual landmarks. However, it was commonly believed that hippocampal neural responses do not depend on which landmark the rat was looking at, a long standing paradox. The other idea is that neurons are keeping track of the distances traveled by the subject, through the so called path-integration, though not directly tested.” Surprisingly, the team found that the neurons did signal for what landmark the rat was looking at, thus removing the 45 year old paradox about whether the landmarks exert a causal influence on hippocampal directional responses. Further, careful experiments using virtual reality showed that the neural responses were neither abstract representations of space, as commonly thought, nor vestibular input driven path-integration signals. Instead, these responses were causally and predictably governed by visual landmarks. This study is a part of series of studies undertaken by the Mehta laboratory to understand how the brain rapidly makes memories, including spatial maps, on the fly. They have been using Virtual Reality to manipulate the perception of space and time. Using this technique they have recently shown that in more than half of the neurons in hippocampus shut down in virtual reality. They also showed that the brain in the virtual world does not create a spatial map like it does in the real world environment, a finding that was replicated in this study, that could have implications for people who use virtual reality for gaming, military, commercial, scientific or other purposes.The scientists were studying the hippocampus, a region of the brain that is critical for learning and memory and involved in diseases such as Alzheimer’s, stroke, schizophrenia, depression, epilepsy and post-traumatic stress disorder. The hippocampus also plays an important role in forming new memories and creating mental maps of space. Researchers created a sophisticated virtual reality environment for rats that cost nearly $1 million to develop. They placed a small harness around rats and put them on a treadmill-like device surrounded by a “virtual world” on large video screens in an otherwise dark, quiet room. The rats walk in the virtual room in a similar way as they would in a real room, Mehta said. Because no mental map was being made, part of the brain was not working in virtual reality. Could there be long-term implications for that as more and more people use virtual reality? “This could have important implications. This part of the brain that makes maps of space is also involved in making memories, like what did I have for lunch, what was I doing on 9/11? It is, in that sense, what makes us human,” Mehta said. “If people are spending time in virtual reality, could that alter the way the brain works? If it’s not making maps, will the making of memories be affected? Everything in the brain influences what the brain will do later on. It’s the reason we are different at age 40 than we were at age four. The brain remembered everything that happened and modified itself because of it. Now what will happen when people’s neural responses become different in virtual reality?”Mehta said the findings, although found in rodents, are equally applicable to humans. 
Credit Newswise —  A physician-researcher at The Children’s Hospital of Philadelphia (CHOP) has received a $1 million Hyundai Quantum Grant from Hyundai Hope on Wheels to advance treatment for a high-risk form of childhood leukemia. The research focuses on immunotherapy—an approach that utilizes a patient’s immune system to better fight off cancer. “This Hyundai Quantum Grant gives us substantial resources to make progress against the most life-threatening form of leukemia,” said CHOP pediatric oncologist Richard Aplenc, M.D., Ph.D. “I’m constantly inspired by the resilience of my patients, and the grace and tenderness of their families, and this grant support is very helpful in advancing new cancer treatments.” The Hyundai Quantum Grant, a new category in Hyundai’s pediatric cancer grant program, provides each research center $250,000 per year for four years. Focusing on pediatric cancers with the lowest survival rates, the program announced competitive awards to CHOP and three other institutions participating in the Children’s Oncology Group. Hyundai Hope on Wheels is the nonprofit organization of Hyundai Motor America, and is in its 18th year of supporting childhood cancer research. This year’s awards are Hyundai’s largest individual research grants ever for pediatric cancer research. “We are proud to fund Dr. Aplenc, one of this year’s Hyundai Quantum winners, and his innovative work in AML,” said Dave Zuchowski, president and CEO, Hyundai Motor America. “We are hopeful for the discoveries to come from his important work in the coming years. No child should ever have to hear the words, ‘You have cancer,’ and we will continue to support the efforts fighting for that day to come.” Overall survival rates for children’s cancer have reached 80 percent, but some high-risk forms of cancer have stubbornly resisted this progress. One such example isacute myeloid leukemia (AML), which has a complicated variety of difficult-to-treat subtypes. Even among survivors of AML, intensive chemotherapy may cause lifelong side effects. Immunotherapy, which is currently being studied in other types of leukemia, may provide a more effective treatment for AML, with fewer long-term side effects. The new grant, said Aplenc, will enable his team to identify specific proteins on the outside surface of AML cells that could be the most appropriate targets for immune cells programmed to attack cancers. “New technologies have dramatically altered the research landscape, by allowing scientists to better identify proteins and to make use of DNA sequencing data from AML patients,” said Aplenc. “This research grant will enable us to discover more about these specific biological molecules, offering data with great potential to help us design innovative treatments.” Stephen Hunger, M.D., chief of the Division of Oncology and director of the Center for Childhood Cancer Researchat CHOP, added, “This award will help CHOP physicians and their collaborators develop new therapies for children with AML that has not responded to current therapies, or has relapsed despite those therapies.” About The Children’s Hospital of Philadelphia: The Children’s Hospital of Philadelphia was founded in 1855 as the nation’s first pediatric hospital. Through its long-standing commitment to providing exceptional patient care, training new generations of pediatric healthcare professionals and pioneering major research initiatives, Children’s Hospital has fostered many discoveries that have benefited children worldwide. Its pediatric research program is among the largest in the country. In addition, its unique family-centered care and public service programs have brought the 535-bed hospital recognition as a leading advocate for children and adolescents. For more information, visit www.chop.edu.
Credit Newswise —  Could there be a vegetarian gene? Cornell University researchers have found evidence of a genetic variation – called an allele – that has evolved in populations that have historically favored vegetarian diets, such as in India, Africa and parts of East Asia. They also discovered a different version of this gene adapted to a marine diet discovered among the Inuit in Greenland, who mainly consume seafood. The vegetarian allele evolved in populations that have eaten a plant-based diet over hundreds of generations. The adaptation allows these people to efficiently process omega-3 and omega-6 fatty acids and convert them into compounds essential for early brain development and if they stray from a balanced omega-6 to omega-3 diet, it may make people more susceptible to inflammation, and by association, increased risk of heart disease and colon cancer. In Inuit populations of Greenland, the researchers uncovered that a previously identified adaptation is opposite to the one found in long-standing vegetarian populations: While the vegetarian allele has an insertion of 22 bases (a base is a building block of DNA) within the gene, this insertion was found to be deleted in the seafood allele. “The opposite allele is likely driving adaptation in Inuit,” said Kaixiong Ye, co-lead author of the paper appearing March 29 in the journal Molecular Biology and Evolution. Ye is a postdoctoral researcher in the lab of Alon Keinan, associate professor of biological statistics and computational biology, and the paper’s co-senior author. “Our study is the first to connect an insertion allele with vegetarian diets, and the deletion allele with a marine diet,” Ye said. “It is the most interesting example of local adaptation that I have been fortunate to help study,” said Keinan. “Several studies have pointed to adaptation in this region of the genome. Our analyses combine to show that the adaptation is driven by an insertion of a small piece of DNA that we know its function. Moreover, when it reached the Greenlandic Inuit, with their marine-based diet rich in omega-3, it might have become detrimental.” FADS1 and FADS2 are enzymes that are essential for converting omega-3 and omega-6 fatty acids into downstream products needed for brain development and controlling inflammation. Meat and seafood eaters have less need for increased FADS1 and FADS2 enzymes to get proper nutrition because their omega-3 and omega-6 fatty acid conversion process is simpler and requires fewer steps. This study is based on previous work by co-senior author Tom Brenna, professor of human nutrition and of chemistry at Cornell University, who showed the insertion can regulate the expression of FADS1 and FADS2 and hypothesized it could be an adaptation in vegetarian populations. Ye, Keinan and colleagues analyzed frequencies of the vegetarian allele in 234 primarily vegetarian Indians and 311 U.S. individuals and found the vegetarian allele in 68 percent of the Indians and in just 18 percent of Americans. Analysis using data from the 1,000 Genomes Project similarly found the vegetarian allele in 70 percent of South Asians, 53 percent of Africans, 29 percent of East Asians and 17 percent of Europeans. “Northern Europeans have a long history of drinking milk and they absorbed enough end products from milk for long-chain fatty acid metabolism so they don’t have to increase capacity to synthesize those fatty acids from precursors,” said Ye. “One implication from our study is that we can use this genomic information to try to tailor our diet so it is matched to our genome, which is called personalized nutrition,” he added. The researchers are not sure yet when the adaptation first occurred, as analyses of chimpanzee or orangutan genomes did not uncover the vegetarian allele. But there is evidence for the allele in early hominid Neanderthal and Denisovan genomes. “It is possible that in the history of human evolution, when people migrated to different environments, sometimes they ate a plant-based diet and sometimes they ate a marine-based diet, and in different time periods these different alleles were adaptive,” meaning the alleles have a tendency to evolve under dietary pressures, Ye said.